Gel Permeation and Size Exclusion Chromatography

Intertek provides chemical characterization by gel permeation chromatography (GPC) and size exclusion chromatography (SEC) for clients across industries and markets. Intertek scientists employ GPC/SEC methods to characterize polymers from aqueous and organic matrices. 

Our GPC and SEC laboratories in Europe and the USA provide critical information to customers, helping speed their development of new polymer synthesis routes, processing schemes and purification techniques.

Gel Permeation and Size Exclusion Chromatography:

• Separation of polymer mixtures by size and shape
• Quantitation of mixture components in various matrices including water and organic liquids
• Measurement of average molecular weight and polydispersity of polymers
• Determination of polymer absolute mass using light scattering
• Provide support data for registering new products as polymers
• High Temperature Gel Permeation Chromatography (HTGPC)
• GPC-NMR Analysis
• Protein Aggregation studies
• Pharmaceutical polymer excipient characterisation

Gel Permeation and Size Exclusion Chromatography Test Methods

• Polymer Evaluation in n-methyl-2-pyrrolidone (NMP) and/or tetrahydrofuran (THF)

  • Low Mass, High Resolution GPC up to 3,000 mass
  • Low Mass GPC up to 30,000 mass
  • Normal Range GPC in range of 500 – 7,500,000 mass
  • Absolute mass determination in Normal Range
   

• Polymer Evaluation in Aqueous Solutions

  • Water-soluble polymers in range of 500 to 1,000,000 mass
   

• Analysis of Amine-based Materials

  • Low Mass GPC up to 50,000 mass
   

• Wyatt Technology

  • Specific refractive index (dn/dc) measurements for light scattering
  • Batch light scattering measurement for molecular weight (Mw)
   
• Wide range of detection methods including refractive index (RI), evaporative light scattering detection (ELSD), charged aerosol detection (CAD), multi-angle laser light-scattering detection (MALLS) and viscosity detectors 
• Standardization of measurements versus polystryrene (PS), polyethylene glycol (PEG), Poly(methyl methacrylate) (PMMA), polythethylene oxide (PEO) and polystyrene sulfonic acid (PSSA)
• Absolute mass using MALLs detection ensures more accurate mass determination, as well as branching information
• Aqueous Gel Permeation Chromatography