Case Study: Determination of Melting Temperature for Oligonucleotide Duplexes using NMR

Oligonucleotide high resolution Nuclear Magnetic Resonance Spectroscopy (NMR) and Topspin Analysis, supporting characterisation, stability and batch release testing

As a critical attribute supporting proof of structure and product characterisation, determination of the Melting Temperature (Tm) for an oligonucleotide therapeutic is essential for product development, supporting identity specification testing. Our scientists apply high resolution NMR to achieve accurate determination of Tm.